Fast and efficient genetic transformation of sugar beet by Agrobacterium rhizogenes
نویسندگان
چکیده
منابع مشابه
A protocol for sonication-assisted Agrobacterium rhizogenes-mediated transformation of haploid and diploid sugar beet (Beta vulgaris L.) explants.
Hairy root cultures obtained after Agrobacterium rhizogenes-mediated genetic transformation can serve as a model system for studying plant metabolism and physiology, or can be utilized for the production of secondary metabolites. So far no efficient protocol of hairy root development in sugar beet has been publically released. In this work, two A. rhizogenes strains (A4T and LBA1334) carrying a...
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The Agrobacterium-mediated transformation of hairy roots creates a rapid and simple means to introduce and express foreign genes in plant cells that are capable of synthesizing specific natural products. We describe a method for the production of transgenic hairy root cultures of peanut (Arachis hypogaea L.) using Agrobacterium rhizogenes R1000, a strain with the binary vector pBI121. We evalua...
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BACKGROUND Plants within the Orobanchaceae are an agriculturally important group of parasites that attack economically important crops to obtain water and nutrients from their hosts. Despite their agricultural importance, molecular mechanisms of the parasitism are poorly understood. METHODOLOGY/PRINCIPAL FINDINGS We developed transient and stable transformation systems for Phtheirospermum jap...
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Agrobacterium tumefaciens and Agrobacterium rhizogenes are related pathogens that cause crown gall and hairy root diseases, which result from integration and expression of bacterial genes in the plant genome. Single-stranded DNA (T strands) and virulence proteins are translocated into plant cells by a type IV secretion system. VirD2 nicks a specific DNA sequence, attaches to the 5' end, and pil...
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Using Agrobacterium rhizogenes due to create hairy roots is a useful method to product secondary metabolites in many medicinal plants. The transgenic hairy roots were induced from Peganum multisectum (Maxim) Bobrov a medicinally important species, by infecting leaf and stem explant with wild type Agrobacterium rhizogenes strain ATTCC 15834, which led to the induction of hairy roots from 19% of ...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2010
ISSN: 2043-0116
DOI: 10.1038/nprot.2010.98